Hotspot

rpl16

LSC · 79375-80830 · 1456 bp

Back to Linum

Pi 0.0596
MarkerSeek score 84.20
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1456
variable_sites
165
pi
0.0596
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GCGATCGCACCAAAAATTCT GATTAGGGAGGGTAGGGTCC 45.0 60.0 57.7 58.0 2157 bp 0.2 45.7
rpl16_p2 GCGATCGCACCAAAAATTCT GGATTAGGGAGGGTAGGGTC 45.0 60.0 57.7 58.0 2158 bp 0.2 45.7
rpl16_p3 AGCAATAGTGTCCCTACCCA GATTAGGGAGGGTAGGGTCC 50.0 60.0 57.7 58.0 2440 bp 0.2 45.7
rpl16_p4 AGCAATAGTGTCCCTACCCA GGATTAGGGAGGGTAGGGTC 50.0 60.0 57.7 58.0 2441 bp 0.2 45.7
rpl16_p5 GCGATCGCACCAAAAATTCT AAAAGAGATTGCACGCGTTG 45.0 45.0 57.7 58.0 2181 bp 0.2 45.6