Hotspot

clpP1

LSC · 67923-70015 · 2093 bp

Back to Linum

Pi 0.218
MarkerSeek score 72.80
Primer available yes
Label clpP1

Feature Metadata

label_name
clpP1
feature_type
gene
region
LSC
length_bp
2093
variable_sites
736
pi
0.218
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.62
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP1_p1 TCTCTTGTCCCAGCTCATGA TCACTTGATTAGCCTGGGGA 50.0 50.0 58.1 58.0 2748-2769 bp 0.4 58.3
clpP1_p2 CCTTTCTCTTGTCCCAGCTC TCACTTGATTAGCCTGGGGA 55.0 50.0 57.9 58.0 2752-2773 bp 0.4 57.9
clpP1_p3 CTAGTACATGTTCCTCGGCG TCACTTGATTAGCCTGGGGA 55.0 50.0 57.9 58.0 2705-2726 bp 0.4 57.8
clpP1_p4 AGTCGCACATACACCCTAGT TCACTTGATTAGCCTGGGGA 50.0 50.0 58.2 58.0 2720-2741 bp 0.4 57.5
clpP1_p5 TCTCTTGTCCCAGCTCATGA ATACCAAGGCAAACCCATGG 50.0 50.0 58.1 58.1 2845-2867 bp 0.4 57.4