Hotspot

accD

LSC · 55046-56515 · 1470 bp

Pi 0.0834

MarkerSeek score 84.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1470
variable_sites: 224
pi: 0.0834
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TAGCGACAAGGGCAAGAAAG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	2120-2600 bp	0.8	82.3
accD_p2	TAACCCTTTCTGCGGTTGTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2724 bp	0.2	46.9
accD_p3	TAACCCTTTCTGCGGTTGTC	AAGGCACAAAAATGGAGGGT	50.0	45.0	58.1	57.9	2691 bp	0.2	46.3
accD_p4	TAACCCTTTCTGCGGTTGTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	2730 bp	0.2	46.3
accD_p5	TCTGCGGTTGTCTTGGATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	2716 bp	0.2	46.3