Hotspot

rps15-trnN-GUU

SSC · 121122-127191 · 6070 bp

Back to Linum

Pi 0.154
MarkerSeek score 78.10
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6070
variable_sites
1633
pi
0.154
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 CCCATGGAGAATGTGGTCAG CCCGGAACTTCTTCGTAGTG 55.0 55.0 58.0 58.0 382 bp 0.4 56.6
rps15-trnN-GUU_p2 CCCATGGAGAATGTGGTCAG GTCTTCGTTCCATGCCTCAT 55.0 50.0 58.0 58.0 363 bp 0.4 56.5
rps15-trnN-GUU_p3 AGGAAGAGATAAGAGGGCCC CCCGGAACTTCTTCGTAGTG 55.0 55.0 57.9 58.0 400 bp 0.4 56.1
rps15-trnN-GUU_p4 CCCATGGAGAATGTGGTCAG ACTCCCGGAACTTCTTCGTA 55.0 50.0 58.0 58.1 385 bp 0.4 56.0
rps15-trnN-GUU_p5 CCCATGGAGAATGTGGTCAG TCCCGGAACTTCTTCGTAGT 55.0 50.0 58.0 58.1 383 bp 0.4 56.0