Hotspot

atpB-rbcL

LSC · 52119-52886 · 768 bp

Back to Linum

Pi 0.101
MarkerSeek score 82.80
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
768
variable_sites
145
pi
0.101
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.93
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TGAATAGGGGATGTTGTGCG CGGTCTCTCCAACGCATAAA 50.0 50.0 58.0 58.0 1795-1953 bp 0.6 73.8
atpB-rbcL_p2 GCCGACTGGAACACTTAGAG CCTGGAATCCAACACTTGCT 55.0 50.0 58.0 58.1 1099-1138 bp 0.4 58.4
atpB-rbcL_p3 GCCGACTGGAACACTTAGAG CGGTCTCTCCAACGCATAAA 55.0 50.0 58.0 58.0 1706-1745 bp 0.4 58.1
atpB-rbcL_p4 AGCTACAGCCCGAACTCTAT CGGTCTCTCCAACGCATAAA 50.0 50.0 57.9 58.0 1631 bp 0.2 47.4
atpB-rbcL_p5 ATAGCTACAGCCCGAACTCT CGGTCTCTCCAACGCATAAA 50.0 50.0 57.9 58.0 1633 bp 0.2 47.4