Hotspot

rpoB-trnC-GCA

LSC · 25524-26645 · 1122 bp

Back to Linum

Pi 0.0858
MarkerSeek score 83.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1122
variable_sites
184
pi
0.0858
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AAAATATTGGATCGCCGCCT GGGAAACATTACGCACAAGC 45.0 50.0 58.0 58.0 2070 bp 0.2 47.7
rpoB-trnC-GCA_p2 CGAGCCCATATCCTTGCTTT GGGAAACATTACGCACAAGC 50.0 50.0 58.0 58.0 1864 bp 0.2 47.6
rpoB-trnC-GCA_p3 CTGATAATAGATGCCGGGGC GGGAAACATTACGCACAAGC 55.0 50.0 58.0 58.0 1757 bp 0.2 47.6
rpoB-trnC-GCA_p4 TGTTCTTGCATATCCCTGCC GGGAAACATTACGCACAAGC 50.0 50.0 57.9 58.0 1642 bp 0.2 47.4
rpoB-trnC-GCA_p5 CCGGGGCTTTGCAATATTTG GGGAAACATTACGCACAAGC 50.0 50.0 58.1 58.0 1744 bp 0.2 47.3