primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AAAATATTGGATCGCCGCCT	GGGAAACATTACGCACAAGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041555	25524	26645	2070-2070	0.200000	47.7
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	GGGAAACATTACGCACAAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049940	25524	26645	1864-1864	0.200000	47.6
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CTGATAATAGATGCCGGGGC	GGGAAACATTACGCACAAGC	20	20	55.0	50.0	58.0	58.0	2.2	0.0	0.052650	25524	26645	1757-1757	0.200000	47.6
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TGTTCTTGCATATCCCTGCC	GGGAAACATTACGCACAAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079949	25524	26645	1642-1642	0.200000	47.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCGGGGCTTTGCAATATTTG	GGGAAACATTACGCACAAGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.084186	25524	26645	1744-1744	0.200000	47.3
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.716274	28201	28673	474-556	1.000000	86.3
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.5	57.8	7.1	0.0	0.717825	28201	28673	473-555	1.000000	86.3
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	28201	28673	495-577	1.000000	85.4
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	28201	28673	494-576	1.000000	85.2
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	20	20	50.0	50.0	58.5	57.5	7.1	0.0	0.998018	28201	28673	490-572	1.000000	84.0
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	58.1	57.0	9.5	29.0	1.054924	33721	34122	451-500	1.000000	83.6
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	CTATCAACCACTCGGCCATC	TGCAAAAACAGCCAATTGGA	20	20	55.0	40.0	58.1	57.0	2.2	29.0	1.125169	33721	34122	440-489	1.000000	83.0
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	CAAGACCGGAGCTATCAACC	TGCAAAAACAGCCAATTGGAA	20	21	55.0	38.1	58.1	57.7	9.5	29.0	1.331249	33721	34122	451-500	1.000000	81.4
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	CAAGACCGGAGCTATCAACC	AAACAGCCAATTGGAAAGCA	20	20	55.0	40.0	58.1	56.7	9.5	29.0	1.356395	33721	34122	446-495	1.000000	81.1
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	CAAGACCGGAGCTATCAACC	AACAGCCAATTGGAAAGCAA	20	20	55.0	40.0	58.1	56.7	9.5	29.0	1.356395	33721	34122	445-494	1.000000	81.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AATGCCGGAAAGCTGGATAG	20	20	55.0	50.0	58.0	58.0	10.3	5.0	0.029633	46020	46776	879-910	0.800000	83.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	CGTTCCCAATGTGCCTATGA	20	20	55.0	50.0	58.0	58.0	10.3	0.0	0.039463	46020	46776	1121-1152	0.800000	83.7
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	ATCTACGTTCCCAATGTGCC	20	20	55.0	50.0	58.0	58.0	10.3	0.0	0.040892	46020	46776	1126-1157	0.400000	62.7
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GGGATAGCTCAGCTGGTAGA	GGGTCCATATCCTCCCATGA	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.047298	46020	46776	1444-1444	0.200000	47.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGGATAGCTCAGCTGGTAGA	AGGGTCCATATCCTCCCATG	20	20	55.0	55.0	58.0	57.9	10.3	0.0	0.053260	46020	46776	1445-1445	0.200000	47.6
ndhC-trnS-ACU_p1	ndhC-trnS-ACU	1	TCTCACTAACGAAGGAGCCA	AGCCTGACAAAAAGTTCGGT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.133542	48453	49096	1876-2389	1.000000	88.1
ndhC-trnS-ACU_p2	ndhC-trnS-ACU	2	TCTCACTAACGAAGGAGCCA	GCACAAGGGCTATAGCTCAG	20	20	50.0	55.0	58.1	58.1	0.0	26.2	0.204438	48453	49096	2020-2538	1.000000	87.5
ndhC-trnS-ACU_p3	ndhC-trnS-ACU	3	TCTCACTAACGAAGGAGCCA	ACAAGCACAAGGGCTATAGC	20	20	50.0	50.0	58.1	58.0	0.0	16.8	0.124882	48453	49096	2024-2047	0.400000	58.1
ndhC-trnS-ACU_p4	ndhC-trnS-ACU	4	TTTAACCAAGCATCGCCCAT	ACAAGCACAAGGGCTATAGC	20	20	45.0	50.0	58.2	58.0	0.0	16.8	0.198116	48453	49096	1506-1527	0.400000	58.1
ndhC-trnS-ACU_p5	ndhC-trnS-ACU	5	TTAACCAAGCATCGCCCATT	ACAAGCACAAGGGCTATAGC	20	20	45.0	50.0	58.2	58.0	0.0	16.8	0.198116	48453	49096	1505-1505	0.200000	46.4
atpB-rbcL_p1	atpB-rbcL	1	TGAATAGGGGATGTTGTGCG	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041540	52119	52886	1795-1953	0.600000	73.8
atpB-rbcL_p2	atpB-rbcL	2	GCCGACTGGAACACTTAGAG	CCTGGAATCCAACACTTGCT	20	20	55.0	50.0	58.0	58.1	0.0	2.6	0.087190	52119	52886	1099-1138	0.400000	58.4
atpB-rbcL_p3	atpB-rbcL	3	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006913	52119	52886	1706-1745	0.400000	58.1
atpB-rbcL_p4	atpB-rbcL	4	AGCTACAGCCCGAACTCTAT	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079311	52119	52886	1631-1631	0.200000	47.4
atpB-rbcL_p5	atpB-rbcL	5	ATAGCTACAGCCCGAACTCT	CGGTCTCTCCAACGCATAAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079311	52119	52886	1633-1633	0.200000	47.4
accD_p1	accD	1	TAGCGACAAGGGCAAGAAAG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	0.0	26.9	0.218593	55046	56515	2120-2600	0.800000	82.3
accD_p2	accD	2	TAACCCTTTCTGCGGTTGTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	1.8	26.9	0.140117	55046	56515	2724-2724	0.200000	46.9
accD_p3	accD	3	TAACCCTTTCTGCGGTTGTC	AAGGCACAAAAATGGAGGGT	20	20	50.0	45.0	58.1	57.9	1.8	0.0	0.214753	55046	56515	2691-2691	0.200000	46.3
accD_p4	accD	4	TAACCCTTTCTGCGGTTGTC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.1	57.9	1.8	26.8	0.216651	55046	56515	2730-2730	0.200000	46.3
accD_p5	accD	5	TCTGCGGTTGTCTTGGATTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	0.0	26.9	0.218593	55046	56515	2716-2716	0.200000	46.3
rpl20-rps12_p1	rpl20-rps12	1	TTCTCCGCCTTCGAGCTATA	ATTTACCGATCTAGGCCCGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028314	66858	67643	1508-1529	0.400000	59.0
rpl20-rps12_p2	rpl20-rps12	2	TTCTCCGCCTTCGAGCTATA	TTTACCGATCTAGGCCCGAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028314	66858	67643	1507-1528	0.400000	59.0
rpl20-rps12_p3	rpl20-rps12	3	TTCTCCGCCTTCGAGCTATA	GGCTTTTATGCGGCACAAAT	20	20	50.0	45.0	58.0	58.0	0.0	7.3	0.031696	66858	67643	1294-1309	0.400000	58.9
rpl20-rps12_p4	rpl20-rps12	4	ACGGCGAAAGTTCCTTTTCT	ATTTACCGATCTAGGCCCGA	20	20	45.0	50.0	58.0	58.0	16.6	0.0	0.042198	66858	67643	1642-1663	0.400000	58.7
rpl20-rps12_p5	rpl20-rps12	5	ACGGCGAAAGTTCCTTTTCT	TTTACCGATCTAGGCCCGAT	20	20	45.0	50.0	58.0	58.0	16.6	0.0	0.042198	66858	67643	1641-1662	0.400000	58.7
clpP1_p1	clpP1	1	TCTCTTGTCCCAGCTCATGA	TCACTTGATTAGCCTGGGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.091810	67923	70015	2748-2769	0.400000	58.3
clpP1_p2	clpP1	2	CCTTTCTCTTGTCCCAGCTC	TCACTTGATTAGCCTGGGGA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.141063	67923	70015	2752-2773	0.400000	57.9
clpP1_p3	clpP1	3	CTAGTACATGTTCCTCGGCG	TCACTTGATTAGCCTGGGGA	20	20	55.0	50.0	57.9	58.0	2.3	0.0	0.155702	67923	70015	2705-2726	0.400000	57.8
clpP1_p4	clpP1	4	AGTCGCACATACACCCTAGT	TCACTTGATTAGCCTGGGGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.185934	67923	70015	2720-2741	0.400000	57.5
clpP1_p5	clpP1	5	TCTCTTGTCCCAGCTCATGA	ATACCAAGGCAAACCCATGG	20	20	50.0	50.0	58.1	58.1	0.0	4.1	0.192555	67923	70015	2845-2867	0.400000	57.4
petD_p1	petD	1	TATCGTCCGACCGTTACTGA	CTTCATGAAGCTTCCCGGAA	20	20	50.0	50.0	58.0	57.9	0.0	22.0	0.136625	74598	75943	2554-2575	0.400000	58.0
petD_p2	petD	2	ACTATCGTCCGACCGTTACT	CTTCATGAAGCTTCCCGGAA	20	20	50.0	50.0	58.0	57.9	0.0	22.0	0.143699	74598	75943	2556-2577	0.400000	57.9
petD_p3	petD	3	GGTGTGGTTCTTGGTGTCTT	CTTCATGAAGCTTCCCGGAA	20	20	50.0	50.0	57.9	57.9	0.0	22.0	0.166643	74598	75943	2365-2386	0.400000	57.9
petD_p4	petD	4	TATCGTCCGACCGTTACTGA	CTTCCCTAGATACACACGCG	20	20	50.0	55.0	58.0	57.9	0.0	3.5	0.143881	74598	75943	2028-2040	0.400000	57.7
petD_p5	petD	5	ACTATCGTCCGACCGTTACT	CTTCCCTAGATACACACGCG	20	20	50.0	55.0	58.0	57.9	0.0	3.5	0.150954	74598	75943	2030-2042	0.400000	57.7
rpl16_p1	rpl16	1	GCGATCGCACCAAAAATTCT	GATTAGGGAGGGTAGGGTCC	20	20	45.0	60.0	57.7	58.0	33.9	0.0	0.289670	79375	80830	2157-2157	0.200000	45.7
rpl16_p2	rpl16	2	GCGATCGCACCAAAAATTCT	GGATTAGGGAGGGTAGGGTC	20	20	45.0	60.0	57.7	58.0	33.9	0.0	0.289670	79375	80830	2158-2158	0.200000	45.7
rpl16_p3	rpl16	3	AGCAATAGTGTCCCTACCCA	GATTAGGGAGGGTAGGGTCC	20	20	50.0	60.0	57.7	58.0	0.0	0.0	0.290993	79375	80830	2440-2440	0.200000	45.7
rpl16_p4	rpl16	4	AGCAATAGTGTCCCTACCCA	GGATTAGGGAGGGTAGGGTC	20	20	50.0	60.0	57.7	58.0	0.0	0.0	0.290993	79375	80830	2441-2441	0.200000	45.7
rpl16_p5	rpl16	5	GCGATCGCACCAAAAATTCT	AAAAGAGATTGCACGCGTTG	20	20	45.0	45.0	57.7	58.0	33.9	10.2	0.299129	79375	80830	2181-2181	0.200000	45.6
ycf2_p1	ycf2	1	CTAATGAAACCGTCGCTGGA	TCCAGAAGGGCATTTAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	4.2	0.024773	85609	85728	788-788	0.400000	56.0
ycf2_p2	ycf2	2	TCTAATGAAACCGTCGCTGG	TCCAGAAGGGCATTTAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	4.2	0.024773	85609	85728	789-789	0.400000	56.0
ycf2_p3	ycf2	3	CTAATGAAACCGTCGCTGGA	CGTGCGGATTATTACCCCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048286	85609	85728	426-426	0.400000	55.8
ycf2_p4	ycf2	4	TCTAATGAAACCGTCGCTGG	CGTGCGGATTATTACCCCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048286	85609	85728	427-427	0.400000	55.8
ycf2_p5	ycf2	5	CTAATGAAACCGTCGCTGGA	GGGTCGCAAAGAATCGATCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.063258	85609	85728	724-724	0.400000	55.7
ndhF-rpl32_p1	ndhF-rpl32	1	GCTGATTTGGCAATTGGACC	AACGCTGCCCAATATCCTTT	20	20	50.0	45.0	58.0	57.9	7.7	0.0	0.158211	109875	110763	1544-1694	0.400000	66.7
ndhF-rpl32_p2	ndhF-rpl32	2	GCTGATTTGGCAATTGGACC	ACGCTGCCCAATATCCTTTT	20	20	50.0	45.0	58.0	57.9	7.7	0.0	0.158211	109875	110763	1543-1693	0.400000	66.7
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGCCCAGATACGACGAC	AAAATGAGAATCCCCACCGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.149846	109875	110763	1377-1377	0.200000	46.8
ndhF-rpl32_p4	ndhF-rpl32	4	AAAAGCCCAGATACGACGAC	AACGCTGCCCAATATCCTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.149846	109875	110763	1101-1101	0.200000	46.8
ndhF-rpl32_p5	ndhF-rpl32	5	AAAAGCCCAGATACGACGAC	ACGCTGCCCAATATCCTTTT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.149846	109875	110763	1100-1100	0.200000	46.8
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	CCCATGGAGAATGTGGTCAG	CCCGGAACTTCTTCGTAGTG	20	20	55.0	55.0	58.0	58.0	15.2	0.7	0.056274	126857	126976	382-382	0.400000	56.6
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	CCCATGGAGAATGTGGTCAG	GTCTTCGTTCCATGCCTCAT	20	20	55.0	50.0	58.0	58.0	15.2	0.0	0.078282	126857	126976	363-363	0.400000	56.5
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	AGGAAGAGATAAGAGGGCCC	CCCGGAACTTCTTCGTAGTG	20	20	55.0	55.0	57.9	58.0	16.5	0.7	0.118583	126857	126976	400-400	0.400000	56.1
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	CCCATGGAGAATGTGGTCAG	ACTCCCGGAACTTCTTCGTA	20	20	55.0	50.0	58.0	58.1	15.2	0.7	0.133303	126857	126976	385-385	0.400000	56.0
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	CCCATGGAGAATGTGGTCAG	TCCCGGAACTTCTTCGTAGT	20	20	55.0	50.0	58.0	58.1	15.2	0.7	0.133303	126857	126976	383-383	0.400000	56.0
rpl23_p1	rpl23	1	GAATGGTTAAAGCGCCCAAC	CACTTGACTGTCTACGGCTC	20	20	50.0	55.0	58.0	58.0	2.3	9.5	0.025228	149996	150277	593-593	0.400000	56.1
rpl23_p2	rpl23	2	GAATGGTTAAAGCGCCCAAC	CAATTCGCCTCTGACCATCA	20	20	50.0	50.0	58.0	58.0	2.3	0.0	0.037621	149996	150277	1231-1231	0.400000	55.9
rpl23_p3	rpl23	3	GAATGGTTAAAGCGCCCAAC	CGCCTCTGACCATCAAATGA	20	20	50.0	50.0	58.0	58.0	2.3	0.0	0.037621	149996	150277	1226-1226	0.400000	55.9
rpl23_p4	rpl23	4	GAATGGTTAAAGCGCCCAAC	TCGCCTCTGACCATCAAATG	20	20	50.0	50.0	58.0	58.0	2.3	0.0	0.037621	149996	150277	1227-1227	0.400000	55.9
rpl23_p5	rpl23	5	GAATGGTTAAAGCGCCCAAC	TCTGTCGGTGCTTGAAACAA	20	20	50.0	45.0	58.0	58.0	2.3	0.0	0.040459	149996	150277	1157-1157	0.400000	55.9