Hotspot

petD

LSC · 76310-77519 · 1210 bp

Back to Linochilus

Pi 0.00173

MarkerSeek score 52.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1210
variable_sites: 20
pi: 0.00173
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1344-1363 bp	1.0	79.3
petD_p2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1343-1362 bp	1.0	79.3
petD_p3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1347-1366 bp	1.0	79.0
petD_p4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1346-1365 bp	1.0	79.0
petD_p5	TTTCCCTTGAACTCCGCAAT	ACTTTGAAGCGACTGTTCCC	45.0	50.0	57.7	58.4	1347-1366 bp	1.0	76.7