Hotspot

ndhD

SSC · 120024-121526 · 1503 bp

Back to Linochilus

Pi 0.00128

MarkerSeek score 35.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 17
pi: 0.00128
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTTTGGTCCAAGCGTATCTT	TCATTATCCAACCGACTCTT	40.0	40.0	55.3	53.8	1583-1593 bp	0.972222	40.3
ndhD_p2	TTTTGGTCCAAGCGTATCTT	TTCATTATCCAACCGACTCT	40.0	40.0	55.3	53.8	1584-1594 bp	0.972222	40.3
ndhD_p3	TTTTTGGTCCAAGCGTATCT	TCATTATCCAACCGACTCTT	40.0	40.0	55.3	53.8	1584-1594 bp	0.972222	40.3
ndhD_p4	TTTTTGGTCCAAGCGTATCT	TTCATTATCCAACCGACTCT	40.0	40.0	55.3	53.8	1585-1595 bp	0.972222	40.3
ndhD_p5	TTTTGGTCCAAGCGTATCTT	TCCAACCGACTCTTAAAAATCA	40.0	36.4	55.3	55.7	1577-1587 bp	0.972222	40.3