Hotspot

trnL-UAG-rpl32

SSC · 122945-123769 · 825 bp

Back to Linochilus

Pi 0.00248

MarkerSeek score 46.40

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 825
variable_sites: 20
pi: 0.00248
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GAAAGGATATTGGGCGTCGT	50.0	50.0	58.0	58.1	951-976 bp	1.0	84.3
trnL-UAG-rpl32_p2	AGCGTGTCTACCGATTTCAC	TGAAAGGATATTGGGCGTCG	50.0	50.0	58.0	58.1	952-977 bp	1.0	84.3
trnL-UAG-rpl32_p3	CAGCGTGTCTACCGATTTCA	GAAAGGATATTGGGCGTCGT	50.0	50.0	58.0	58.1	952-977 bp	1.0	84.3
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	TGAAAGGATATTGGGCGTCG	50.0	50.0	58.0	58.1	953-978 bp	1.0	84.2
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GAAAGGATATTGGGCGTCGT	50.0	50.0	57.9	58.1	961-986 bp	1.0	83.3