Hotspot

psaC-ndhD

SSC · 119913-120023 · 111 bp

Back to Linochilus

Pi 0.0252

MarkerSeek score 55.60

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 111
variable_sites: 7
pi: 0.0252
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	AAGAACGGAGGATTGTGTCG	AGGAACCCGCAAATATTGGT	50.0	45.0	57.9	57.5	286-288 bp	1.0	77.4
psaC-ndhD_p2	CCAAGAACGGAGGATTGTGT	AGGAACCCGCAAATATTGGT	50.0	45.0	57.8	57.5	288-290 bp	1.0	76.5
psaC-ndhD_p3	AAGAGATGTGAATCCGCCTG	AGGAACCCGCAAATATTGGT	50.0	45.0	57.7	57.5	261-263 bp	1.0	75.7
psaC-ndhD_p4	CGCAGCATGGGTATAGCTTA	AGGAACCCGCAAATATTGGT	50.0	45.0	57.5	57.5	192-194 bp	1.0	75.5
psaC-ndhD_p5	AAGAACGGAGGATTGTGTCG	AAGGAACCCGCAAATATTGGT	50.0	42.9	57.9	58.2	287-289 bp	1.0	72.1