Hotspot

clpP-psbB

LSC · 71721-72220 · 500 bp

Back to Linochilus

Pi 0.00264

MarkerSeek score 53.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 10
pi: 0.00264
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GTTGCATCTTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.0	58.2	539-608 bp	1.0	83.2
clpP-psbB_p2	GTTGCATCTTCCTCTCCAGG	AGCAACCAAAGCTGTATGCA	55.0	45.0	58.0	58.4	612-681 bp	1.0	81.9
clpP-psbB_p3	ACCCAAGTTGCATCTTCCTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	545-614 bp	1.0	81.8
clpP-psbB_p4	GTTGCATCTTCCTCTCCAGG	ACACGATACCAAGGCAAACC	55.0	50.0	58.0	58.5	542-611 bp	1.0	81.4
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	530-599 bp	1.0	81.1