Hotspot

accD-psaI

LSC · 58224-59112 · 889 bp

Back to Linochilus

Pi 0.0093

MarkerSeek score 65.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 889
variable_sites: 67
pi: 0.0093
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	768-1054 bp	1.0	85.1
accD-psaI_p2	AAAGGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	767-1053 bp	1.0	85.1
accD-psaI_p3	AAAAGGCGTTCTGAGTGAGT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	758-1044 bp	0.972222	81.9
accD-psaI_p4	AAAGGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	757-1043 bp	0.972222	81.9
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	AGGCCTACTAAAGGGACCAA	45.0	50.0	57.4	57.6	737-1023 bp	0.972222	81.1