Hotspot

trnF-GAA-ndhJ

LSC · 48049-48705 · 657 bp

Back to Linochilus

Pi 0.00323

MarkerSeek score 52.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 17
pi: 0.00323
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AATCCTCGTGTCACCAGTTC	TGGATAGGATGGCCTTTACG	50.0	50.0	57.5	56.8	747-759 bp	1.0	68.8
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	GGATAGGATGGCCTTTACGT	50.0	50.0	57.5	56.8	746-758 bp	1.0	68.8
trnF-GAA-ndhJ_p3	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGATGGCC	50.0	47.6	57.5	57.7	754-766 bp	1.0	68.2
trnF-GAA-ndhJ_p4	TGGTAGAGCAGAGGACTGAA	TGGATAGGATGGCCTTTACG	50.0	50.0	57.4	56.8	767-779 bp	1.0	67.6
trnF-GAA-ndhJ_p5	TTGGTAGAGCAGAGGACTGA	TGGATAGGATGGCCTTTACG	50.0	50.0	57.4	56.8	768-780 bp	1.0	67.6