Hotspot

atpA-trnR-UCU

LSC · 29565-29699 · 135 bp

Back to Linochilus

Pi 0.00861

MarkerSeek score 55.70

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 8
pi: 0.00861
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TTTACCGAGGAAGCAGAAGC	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.9	58.3	280-306 bp	1.0	75.0
atpA-trnR-UCU_p2	TACTTCAGGAACAAGCAGCC	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.8	58.3	219-245 bp	1.0	74.9
atpA-trnR-UCU_p3	TTTACCGAGGAAGCAGAAGC	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.9	56.6	280-306 bp	1.0	74.2
atpA-trnR-UCU_p4	CAGGAACAGAGGGAACGTTT	GCAATGAAAAGCGTCCATTGT	50.0	42.9	57.7	58.3	241-267 bp	1.0	74.1
atpA-trnR-UCU_p5	TACTTCAGGAACAAGCAGCC	GCAATGAAAAGCGTCCATTG	50.0	45.0	57.8	56.6	219-245 bp	1.0	74.1