Hotspot

rps16-trnQ-UUG

LSC · 6187-7159 · 973 bp

Back to Linochilus

Pi 0.0028
MarkerSeek score 55.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
973
variable_sites
27
pi
0.0028
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1040-1071 bp 1.0 81.2
rps16-trnQ-UUG_p2 TGCTTTCTACCGCATCGTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 1029-1060 bp 1.0 80.8
rps16-trnQ-UUG_p3 TTGCTTTCTACCGCATCGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 1030-1061 bp 1.0 80.8
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1055-1086 bp 1.0 79.4
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1054-1085 bp 1.0 79.4