Hotspot

rpl32-trnL-UAG

SSC · 112789-113605 · 817 bp

Back to Lindernia

Pi 0.0822

MarkerSeek score 83.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 817
variable_sites: 123
pi: 0.0822
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATGAATTAGGAAAGCGGCGT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1113-1130 bp	1.0	90.3
rpl32-trnL-UAG_p2	ATGAATTAGGAAAGCGGCGT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1114-1131 bp	1.0	90.3
rpl32-trnL-UAG_p3	ACGAAAGGTATGATCCACGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1582-1591 bp	0.4	59.3
rpl32-trnL-UAG_p4	CGAAAGGTATGATCCACGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1581-1590 bp	0.4	59.2
rpl32-trnL-UAG_p5	ACGAAAGGTATGATCCACGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1583-1592 bp	0.4	59.2