Hotspot

petA-psbJ

LSC · 64261-65302 · 1042 bp

Back to Lindernia

Pi 0.0487

MarkerSeek score 82.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 97
pi: 0.0487
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTATCCCGACGGAAGCAAG	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1800-1820 bp	1.0	87.0
petA-psbJ_p2	TTTATCCCGACGGAAGCAAG	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	2039-2059 bp	1.0	86.2
petA-psbJ_p3	TCAGAGCTATCGCCCCAATA	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1970-1981 bp	0.6	70.0
petA-psbJ_p4	TCAGAGCTATCGCCCCAATA	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	2209-2220 bp	0.6	69.4
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	2298-2309 bp	0.6	69.2