Hotspot

accD-psaI

LSC · 60182-60630 · 449 bp

Back to Lindernia

Pi 0.0795

MarkerSeek score 76.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 57
pi: 0.0795
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	CATTTAAGGCCTCCAGCGAT	55.0	50.0	58.0	58.0	873 bp	0.2	47.4
accD-psaI_p2	GCCGAACCCAATTCCTACAT	CATTTAAGGCCTCCAGCGAT	50.0	50.0	57.9	58.0	974 bp	0.2	47.4
accD-psaI_p3	GACAGGATTAACCGAGGCTG	CATTTAAGGCCTCCAGCGAT	55.0	50.0	58.1	58.0	1329 bp	0.2	47.3
accD-psaI_p4	CCAGAAGGGCTTATTCGACC	GTTCGGCGGAAATCCTATGT	55.0	50.0	58.0	58.1	776 bp	0.2	47.2
accD-psaI_p5	CCAGAAGGGCTTATTCGACC	TGTTCGGCGGAAATCCTATG	55.0	50.0	58.0	58.1	777 bp	0.2	47.2