Hotspot

trnS-GGA-rps4

LSC · 46302-46580 · 279 bp

Back to Lindernia

Pi 0.0556

MarkerSeek score 82.60

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 279
variable_sites: 29
pi: 0.0556
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	AGTAAATCGGTTGGTTTGAA	50.0	35.0	58.1	53.0	380-400 bp	1.0	50.0
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	AGTAAATCGGTTGGTTTGAAA	50.0	33.3	58.1	53.9	380-400 bp	1.0	50.0
trnS-GGA-rps4_p3	AGGCTTTTGTTTACCGAGGG	AGTAAATCGGTTGGTTTGAAAA	50.0	31.8	58.1	54.8	380-400 bp	1.0	50.0
trnS-GGA-rps4_p4	GGCTTTTGTTTACCGAGGGT	AGTAAATCGGTTGGTTTGAA	50.0	35.0	58.4	53.0	379-399 bp	1.0	50.0
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	AGATAGTAAATCGGTTGGTTTGA	50.0	34.8	58.1	55.7	384-404 bp	1.0	49.9