Hotspot

atpH-atpI

LSC · 14186-15168 · 983 bp

Back to Lindernia

Pi 0.0426

MarkerSeek score 82.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 81
pi: 0.0426
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1040-1109 bp	1.0	85.0
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1057-1126 bp	1.0	84.0
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.3	58.5	1052-1121 bp	1.0	83.7
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.1	57.3	1025-1094 bp	1.0	83.4
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.6	58.5	1053-1122 bp	1.0	82.4