Hotspot

trnS-GCU-trnG-CGA

LSC · 8901-9563 · 663 bp

Back to Lindernia

Pi 0.0517
MarkerSeek score 86.60
Primer available yes
Label trnS-GCU-trnG-CGA

Feature Metadata

label_name
trnS-GCU-trnG-CGA
feature_type
igs
region
LSC
length_bp
663
variable_sites
66
pi
0.0517
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-CGA_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 721-802 bp 1.0 60.6
trnS-GCU-trnG-CGA_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 720-801 bp 1.0 60.6
trnS-GCU-trnG-CGA_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 724-805 bp 1.0 59.5
trnS-GCU-trnG-CGA_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 723-804 bp 1.0 59.5
trnS-GCU-trnG-CGA_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 722-803 bp 1.0 59.5