Hotspot

trnK-UUU-rps16

LSC · 4444-5350 · 907 bp

Back to Lindernia

Pi 0.0609

MarkerSeek score 82.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 102
pi: 0.0609
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGGATCAGTCGTGGTCTT	AAGATCTTCGAAACGTGGGG	50.0	50.0	58.1	57.9	1150-1204 bp	1.0	90.1
trnK-UUU-rps16_p2	TTCAGGATCAGTCGTGGTCT	AAGATCTTCGAAACGTGGGG	50.0	50.0	58.1	57.9	1151-1205 bp	1.0	90.1
trnK-UUU-rps16_p3	CCTTTCAGGATCAGTCGTGG	AAGATCTTCGAAACGTGGGG	55.0	50.0	58.0	57.9	1208 bp	0.4	59.6
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	AGCCGTCTATCGAATCGTTG	55.0	50.0	58.0	57.9	1258 bp	0.4	59.2
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	TCGATCCCGAAGAGAAGGAA	55.0	50.0	58.0	57.9	1228 bp	0.4	59.2