Hotspot

ycf2

LSC · 90054-96890 · 6837 bp

Back to Lindera

Pi 0.00575

MarkerSeek score 58.10

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6837
variable_sites: 238
pi: 0.00575
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CGATTGATTGGTCCGAGGTT	TACTTCCCCCTTCTCAACGT	50.0	50.0	58.0	58.0	1076-1082 bp	1.0	85.4
ycf2_p2	ATCTCCATTCATAGGGCCGA	TACTTCCCCCTTCTCAACGT	50.0	50.0	58.0	58.0	933-939 bp	0.96	83.6
ycf2_p3	TCCAAGCCGCTTCTCTTTTT	TACTTCCCCCTTCTCAACGT	45.0	50.0	58.0	58.0	993-999 bp	0.76	74.2
ycf2_p4	TCCAAGCCGCTTCTCTTTTT	TGCCAGATACCTGTGACTCA	45.0	50.0	58.0	58.1	646-652 bp	0.76	74.2
ycf2_p5	ACAACATAATGGAGGCGGTC	TACTTCCCCCTTCTCAACGT	50.0	50.0	58.0	58.0	1493 bp	0.36	55.6