Hotspot

ndhD

SSC · 120081-121589 · 1509 bp

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Pi 0.00572

MarkerSeek score 44.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1509
variable_sites: 45
pi: 0.00572
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GAGGCATGGGGGTCTTATTG	TCTGCTCCAAGAACAGAGGA	55.0	50.0	58.0	58.0	2216-2236 bp	1.0	83.1
ndhD_p2	GTGTGGATTCGGCAATTGTG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2107-2127 bp	1.0	83.1
ndhD_p3	GAGGCATGGGGGTCTTATTG	ATTCGTCTGGCGTCTACAAC	55.0	50.0	58.0	58.0	2428-2448 bp	1.0	83.0
ndhD_p4	GTGTGGATTCGGCAATTGTG	ATTCGTCTGGCGTCTACAAC	50.0	50.0	58.0	58.0	2319-2339 bp	1.0	83.0
ndhD_p5	GAGGCATGGGGGTCTTATTG	CTATTGGGCCGGCTATTGTT	55.0	50.0	58.0	58.0	2686-2723 bp	1.0	83.0