Hotspot

ndhF-rpl32

SSC · 116043-117151 · 1109 bp

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Pi 0.0146

MarkerSeek score 79.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1109
variable_sites: 101
pi: 0.0146
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1221-1362 bp	1.0	87.8
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1215-1356 bp	1.0	87.8
ndhF-rpl32_p3	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1279-1420 bp	0.96	84.1
ndhF-rpl32_p4	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1273-1414 bp	0.96	84.0
ndhF-rpl32_p5	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.3	57.7	1216-1357 bp	0.92	82.2