Hotspot

ycf1-ndhF

SSC · 113795-113819 · 25 bp

Back to Lindera

Pi 0.0794

MarkerSeek score 69.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 25
variable_sites: 7
pi: 0.0794
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AGGTACAGCTTGAGCAATGA	GGGAGGGCGAATCTCTTCTT	45.0	55.0	56.8	59.2	218-250 bp	1.0	69.6
ycf1-ndhF_p2	AAGGTACAGCTTGAGCAATGA	GGGAGGGCGAATCTCTTCTT	42.9	55.0	57.6	59.2	219-251 bp	1.0	67.5
ycf1-ndhF_p3	AGGTACAGCTTGAGCAATGAA	GGGAGGGCGAATCTCTTCTT	42.9	55.0	57.6	59.2	218-250 bp	1.0	67.5
ycf1-ndhF_p4	AGGTACAGCTTGAGCAATGA	GGGAGGGCGAATCTCTTCT	45.0	57.9	56.8	58.5	218-250 bp	1.0	67.1
ycf1-ndhF_p5	AGGTACAGCTTGAGCAATGA	AGGGATCAAATATGTGGGGG	45.0	50.0	56.8	56.6	243-268 bp	0.92	62.0