Hotspot

petA-psbJ

LSC · 66427-67564 · 1138 bp

Back to Lindera

Pi 0.0191

MarkerSeek score 73.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 97
pi: 0.0191
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATCCTAGCACA	ATTCCGGATTGGGATCATCC	47.6	50.0	57.5	57.1	1225-1294 bp	1.0	70.5
petA-psbJ_p2	TGGCATCTGTTATCCTAGCAC	ATTCCGGATTGGGATCATCC	47.6	50.0	57.5	57.1	1226-1295 bp	1.0	70.5
petA-psbJ_p3	GGTTTGTTGTTCTTCTTGGCA	ATTCCGGATTGGGATCATCC	42.9	50.0	57.5	57.1	1242-1311 bp	1.0	70.1
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGATCATCC	42.9	50.0	57.2	57.1	1174-1243 bp	1.0	68.6
petA-psbJ_p5	AGGTTTGTTGTTCTTCTTGGC	ATTCCGGATTGGGATCATCC	42.9	50.0	57.2	57.1	1243-1312 bp	1.0	67.8