Hotspot

ndhC-trnV-UAC

LSC · 52671-54529 · 1859 bp

Back to Lindera

Pi 0.00797

MarkerSeek score 67.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1859
variable_sites: 84
pi: 0.00797
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTTCACTAATCGGGGCCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1965-2016 bp	1.0	84.9
ndhC-trnV-UAC_p2	ACTAATCGGGGCCAAAACTC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1961-2012 bp	1.0	84.9
ndhC-trnV-UAC_p3	CACTAATCGGGGCCAAAACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1962-2013 bp	1.0	84.6
ndhC-trnV-UAC_p4	TCACTAATCGGGGCCAAAAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1963-2014 bp	1.0	84.5
ndhC-trnV-UAC_p5	CTTCACTAATCGGGGCCAAA	ACGCATGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	2031-2082 bp	1.0	83.8