Hotspot

trnS-GGA-rps4

LSC · 47589-47875 · 287 bp

Back to Lindera

Pi 0.0228

MarkerSeek score 65.20

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 287
variable_sites: 24
pi: 0.0228
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTCGAATCCCTCTCTTTCCG	ATTATTCCCGTCAGACTTGA	50.0	40.0	57.0	53.8	313-334 bp	1.0	50.0
trnS-GGA-rps4_p2	GGGTTCGAATCCCTCTCTTT	ATTATTCCCGTCAGACTTGA	50.0	40.0	56.9	53.8	316-337 bp	1.0	49.9
trnS-GGA-rps4_p3	GGGTTCGAATCCCTCTCTTTC	ATTATTCCCGTCAGACTTGA	52.4	40.0	58.1	53.8	316-337 bp	1.0	49.9
trnS-GGA-rps4_p4	GGTTCGAATCCCTCTCTTTCC	ATTATTCCCGTCAGACTTGA	52.4	40.0	58.1	53.8	315-336 bp	1.0	49.9
trnS-GGA-rps4_p5	CGAGTGGTTCAAGGCGTAG	ATTATTCCCGTCAGACTTGA	57.9	40.0	58.3	53.8	369-390 bp	1.0	49.1