Hotspot

psbM-trnD-GUC

LSC · 31383-32355 · 973 bp

Back to Lindera

Pi 0.0125

MarkerSeek score 66.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 67
pi: 0.0125
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	58.2	57.9	1081-1110 bp	1.0	76.0
psbM-trnD-GUC_p2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	56.7	57.9	1080-1109 bp	1.0	75.8
psbM-trnD-GUC_p3	AGAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	42.9	50.0	58.2	57.9	1110-1139 bp	1.0	75.4
psbM-trnD-GUC_p4	GAATGAACAACGCAGTAGCA	ATGCTGTACACCCCATTTCC	45.0	50.0	56.7	57.9	1109-1138 bp	1.0	75.3
psbM-trnD-GUC_p5	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	57.5	57.9	1080-1109 bp	1.0	73.6