Hotspot

petN-psbM

LSC · 30213-31277 · 1065 bp

Back to Lindera

Pi 0.00476

MarkerSeek score 62.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1065
variable_sites: 34
pi: 0.00476
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	58.2	58.2	1223-1243 bp	1.0	71.8
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGTTGTTCATTC	50.0	45.0	58.2	56.7	1223-1243 bp	1.0	71.7
petN-psbM_p3	GGGACACGATTCACATGGAT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	57.7	58.2	1244-1264 bp	1.0	70.4
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCGTTGTTCATTCT	50.0	42.9	57.6	58.2	1162-1182 bp	1.0	70.3
petN-psbM_p5	GGGACACGATTCACATGGAT	TGCTACTGCGTTGTTCATTC	50.0	45.0	57.7	56.7	1244-1264 bp	1.0	70.2