Hotspot

atpH-atpI

LSC · 15013-15688 · 676 bp

Back to Lindera

Pi 0.00955

MarkerSeek score 72.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 38
pi: 0.00955
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	747-766 bp	1.0	85.5
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	803-822 bp	1.0	84.5
atpH-atpI_p3	GATACCTTCTACGGCTTGGC	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.1	57.9	832-851 bp	1.0	84.1
atpH-atpI_p4	CGATACCTTCTACGGCTTGG	TTTTGCAACTTTAGCCGCAG	55.0	45.0	57.9	57.9	833-852 bp	1.0	84.0
atpH-atpI_p5	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	739-758 bp	0.92	81.8