Hotspot

rps16-trnQ-UUG

LSC · 6299-8175 · 1877 bp

Back to Lindera

Pi 0.0097
MarkerSeek score 64.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1877
variable_sites
120
pi
0.0097
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1939-1981 bp 1.0 86.2
rps16-trnQ-UUG_p2 GGGTACAAGAATCCACAGCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.2 58.1 1972-2014 bp 1.0 85.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1943-1985 bp 1.0 85.1
rps16-trnQ-UUG_p4 GGGTACAAGAATCCACAGCC CTCGGAGGTTCGAATCCTTC 55.0 55.0 58.2 57.8 1976-2018 bp 1.0 84.1
rps16-trnQ-UUG_p5 GGTACAAGAATCCACAGCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1971-2013 bp 1.0 83.3