Hotspot

ycf1

SSC · 135558-141116 · 5559 bp

Back to Lindenbergia

Pi 0.343

MarkerSeek score 75.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5559
variable_sites: 1846
pi: 0.343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCGTGCTCTCACAACAAAA	AACGACTGCCATTGTTCGTA	45.0	45.0	58.0	57.8	799 bp	0.5	58.5
ycf1_p2	GAGATCTTGGATCCGTGCTC	AACGACTGCCATTGTTCGTA	55.0	45.0	57.9	57.8	810 bp	0.5	57.6
ycf1_p3	GATCTTGGATCCGTGCTCTC	AACGACTGCCATTGTTCGTA	55.0	45.0	57.9	57.8	808 bp	0.5	57.6
ycf1_p4	GATCCGTGCTCTCACAACAA	AACGACTGCCATTGTTCGTA	50.0	45.0	58.2	57.8	801 bp	0.5	57.1
ycf1_p5	TATTGAGGCCTGGATCACGA	AACGACTGCCATTGTTCGTA	50.0	45.0	58.2	57.8	1226 bp	0.5	57.0