Hotspot

ndhH

SSC · 141892-143073 · 1182 bp

Back to Lindenbergia

Pi 0.432

MarkerSeek score 81.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 498
pi: 0.432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CTTTCAGCAATGCACGGAAG	CCATCTGCTAGAGCTTGCAA	50.0	50.0	58.0	58.0	1458 bp	0.5	59.5
ndhH_p2	CTTTCAGCAATGCACGGAAG	CATAAGAAGTCCAACGGGGG	50.0	55.0	58.0	58.0	1663 bp	0.5	59.5
ndhH_p3	CTTTCAGCAATGCACGGAAG	AGAGCTTGCAAAAGTCCCAA	50.0	45.0	58.0	57.9	1449 bp	0.5	59.2
ndhH_p4	CTTTCAGCAATGCACGGAAG	CGTTGTTGTATCCCTGCAGA	50.0	50.0	58.0	57.9	1404 bp	0.5	59.0
ndhH_p5	CAGCAATGCACGGAAGAATG	CCATCTGCTAGAGCTTGCAA	50.0	50.0	58.1	58.0	1454 bp	0.5	59.0