Hotspot

ndhF

SSC · 151871-154129 · 2259 bp

Back to Lindenbergia

Pi 0.425

MarkerSeek score 78.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 945
pi: 0.425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAAAACGCCTTTAACGCTG	TCTTTTCGATTCGAAGCGGT	50.0	45.0	58.3	57.9	2865 bp	0.5	56.9
ndhF_p2	GATCCATTAGGCCGGAAACA	TCTTTTCGATTCGAAGCGGT	50.0	45.0	57.7	57.9	2369 bp	0.5	56.7
ndhF_p3	GGAAAACGCCTTTAACGCTG	CTTTTCGATTCGAAGCGGTG	50.0	50.0	58.3	57.9	2864 bp	0.5	56.3
ndhF_p4	AAAACGCCTTTAACGCTGTC	TCTTTTCGATTCGAAGCGGT	45.0	45.0	57.6	57.9	2863 bp	0.5	56.2
ndhF_p5	GAAAACGCCTTTAACGCTGT	TCTTTTCGATTCGAAGCGGT	45.0	45.0	57.6	57.9	2864 bp	0.5	56.2