Hotspot

ndhD

SSC · 147769-149274 · 1506 bp

Back to Lindenbergia

Pi 0.432

MarkerSeek score 80.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 641
pi: 0.432
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGTTGGGCTGGCTATTGTTT	TTTGTGTGAACTGGCGAGAA	45.0	45.0	58.0	58.0	2411 bp	0.5	59.7
ndhD_p2	CGGGTTTGATACGGGTAAGG	TTTGTGTGAACTGGCGAGAA	55.0	45.0	58.1	58.0	2272 bp	0.5	59.3
ndhD_p3	TGTTGGGCTGGCTATTGTTT	TTGGAGTTGGGACCCAAAAG	45.0	50.0	58.0	57.9	2700 bp	0.5	59.3
ndhD_p4	TGTTGGGCTGGCTATTGTTT	CTAACGAAGCATGGGGATCC	45.0	55.0	58.0	58.1	2722 bp	0.5	59.0
ndhD_p5	ACAAATCGCTTCTGCTCCAA	TTTGTGTGAACTGGCGAGAA	45.0	45.0	58.1	58.0	1963 bp	0.5	58.9