Hotspot

trnT-GGU-psbD

LSC · 57872-59099 · 1228 bp

Back to Lindenbergia

Pi 0.0644
MarkerSeek score 81.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1228
variable_sites
74
pi
0.0644
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1274-1284 bp 1.0 48.6
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1296-1306 bp 1.0 48.6
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1297-1307 bp 1.0 48.5
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1300-1310 bp 1.0 48.5
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.1 52.8 1299-1309 bp 1.0 48.5