Hotspot

trnK-UUU-rps16

LSC · 30286-31212 · 927 bp

Back to Lindenbergia

Pi 0.102
MarkerSeek score 83.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
927
variable_sites
86
pi
0.102
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG TTTTCTTGAGCCGTACGAGG 55.0 50.0 58.0 57.9 1347-1360 bp 1.0 88.6
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TTTTCTTGAGCCGTACGAGG 55.0 50.0 58.0 57.9 1346-1359 bp 1.0 88.6
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1338-1351 bp 1.0 88.6
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1337-1350 bp 1.0 88.6
trnK-UUU-rps16_p5 TCCCTTTCAGGATCAGTCGT TTTTCTTGAGCCGTACGAGG 50.0 50.0 58.1 57.9 1348-1361 bp 1.0 88.2