primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	TGTTGGGGCGAATCAGAAAA	GAGCTTAGTTTCCGTCTGGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.023247	25917	26477	1623-1623	0.500000	59.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	TGTTGGGGCGAATCAGAAAA	GAGTGGGAGCTTAGTTTCCG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.023247	25917	26477	1629-1629	0.500000	59.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	TGTTGGGGCGAATCAGAAAA	GGAGCTTAGTTTCCGTCTGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.023247	25917	26477	1624-1624	0.500000	59.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	TGTTGGGGCGAATCAGAAAA	GGGAGCTTAGTTTCCGTCTG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.023247	25917	26477	1625-1625	0.500000	59.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GGTTATCCTGCACTCGGAAG	GAGCTTAGTTTCCGTCTGGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.070215	25917	26477	1463-1463	0.500000	59.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCCTTTCAGGATCAGTCGTG	TTTTCTTGAGCCGTACGAGG	20	20	55.0	50.0	58.0	57.9	0.0	18.8	0.090283	30286	31212	1347-1360	1.000000	88.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	TTTTCTTGAGCCGTACGAGG	20	20	55.0	50.0	58.0	57.9	0.0	18.8	0.090283	30286	31212	1346-1359	1.000000	88.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCCTTTCAGGATCAGTCGTG	GCCGTACGAGGAGAAAACTT	20	20	55.0	50.0	58.0	57.9	0.0	18.8	0.091715	30286	31212	1338-1351	1.000000	88.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	20	20	55.0	50.0	58.0	57.9	0.0	18.8	0.091715	30286	31212	1337-1350	1.000000	88.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCCTTTCAGGATCAGTCGT	TTTTCTTGAGCCGTACGAGG	20	20	50.0	50.0	58.1	57.9	0.0	18.8	0.135962	30286	31212	1348-1361	1.000000	88.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	32348	33122	865-1236	1.000000	83.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	32348	33122	848-1219	1.000000	83.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	32348	33122	866-1237	1.000000	82.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AGCACCCTCATTCCTAGACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.0	16.9	0.330253	32348	33122	913-1284	1.000000	82.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	32348	33122	862-1233	1.000000	81.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	34428	35218	824-860	1.000000	61.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	34428	35218	823-859	1.000000	61.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCTTTAACT	20	20	50.0	40.0	58.0	54.5	0.0	0.0	3.519820	34428	35218	861-897	1.000000	61.1
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCTTTAACT	20	20	50.0	40.0	58.0	54.5	0.0	0.0	3.521208	34428	35218	860-896	1.000000	61.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAATCCGACGCTTTAGTCCA	AAACCGAAAGACCCTTTAACT	20	21	50.0	38.1	58.0	55.3	0.0	0.0	3.668017	34428	35218	862-898	1.000000	59.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGGGTCGTGCTCTATCAAAA	CCCAATATATGACTCGCCCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.014728	54311	55128	2037-2037	0.500000	59.9
trnC-GCA-petN_p2	trnC-GCA-petN	2	CGGGTCGTGCTCTATCAAAA	AATCCGGGCATCGCTAAAAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023326	54311	55128	1742-1742	0.500000	59.8
trnC-GCA-petN_p3	trnC-GCA-petN	3	CGGGTCGTGCTCTATCAAAA	AGGTCCGGTTTTCCTCAGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024390	54311	55128	1883-1883	0.500000	59.8
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	CCCAATATATGACTCGCCCG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.092273	54311	55128	1435-1435	0.500000	59.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	AATCCGGGCATCGCTAAAAT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.100871	54311	55128	1140-1140	0.500000	59.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.3	53.0	0.0	6.7	5.273069	57872	59099	1274-1284	1.000000	48.6
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	20	20	55.0	40.0	58.1	52.8	7.6	6.7	5.305690	57872	59099	1296-1306	1.000000	48.6
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	20	20	55.0	40.0	58.1	53.0	7.6	6.7	5.054330	57872	59099	1297-1307	1.000000	48.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.1	53.0	0.0	6.7	5.076485	57872	59099	1300-1310	1.000000	48.5
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTATAGTCAT	20	20	50.0	40.0	58.1	52.8	0.0	6.7	5.327845	57872	59099	1299-1309	1.000000	48.5
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	70707	71455	838-854	1.000000	78.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	70707	71455	809-825	1.000000	77.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	70707	71455	859-875	1.000000	77.5
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	70707	71455	839-855	1.000000	75.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.6	58.1	8.2	0.0	1.514239	70707	71455	836-852	1.000000	75.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	72867	73595	771-790	1.000000	85.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	72867	73595	803-822	1.000000	84.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AACATAGGAAAAAGCCGGCT	CCGTAGCGTCTACCGATTTC	20	20	45.0	55.0	57.8	58.2	27.1	10.2	0.464391	72867	73595	826-845	1.000000	84.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	72867	73595	765-784	1.000000	84.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	72867	73595	822-841	1.000000	84.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	CGTAAGGATTATATTGCCCCCA	20	22	55.0	45.5	58.0	57.7	10.3	0.0	2.297646	74571	75213	764-783	1.000000	69.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	CGTAAGGATTATATTGCCCCCA	20	22	50.0	45.5	58.1	57.7	0.0	0.0	2.347036	74571	75213	728-747	1.000000	69.5
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	CGTAAGGATTATATTGCCCCCA	20	22	55.0	45.5	57.9	57.7	2.5	0.0	2.431439	74571	75213	752-771	1.000000	68.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	TACATTGGGAATGGTCGGGA	CGTAAGGATTATATTGCCCCCA	20	22	50.0	45.5	58.4	57.7	0.0	0.0	2.709246	74571	75213	780-799	1.000000	66.0
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GAATGGTCGGGATAGCTCAG	CGTAAGGATTATATTGCCCCCA	20	22	55.0	45.5	57.6	57.7	0.0	0.0	2.742385	74571	75213	772-791	1.000000	65.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCGGAAATGAAAAATGCCAA	GTTCGAGTCCGTATAGCCCT	20	20	40.0	55.0	55.4	58.7	0.0	0.0	3.281420	76885	78001	1224-1235	1.000000	61.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCGGAAATGAAAAATGCCAAA	GTTCGAGTCCGTATAGCCCT	21	20	38.1	55.0	56.2	58.7	0.0	0.0	3.491317	76885	78001	1224-1235	1.000000	59.7
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCGGAAATGAAAAATGCCAA	TTCGAGTCCGTATAGCCCTA	20	20	40.0	50.0	55.4	57.1	0.0	0.0	3.534990	76885	78001	1223-1234	1.000000	59.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCGGAAATGAAAAATGCCAAA	TTCGAGTCCGTATAGCCCTA	21	20	38.1	50.0	56.2	57.1	0.0	0.0	3.744887	76885	78001	1223-1234	1.000000	57.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCGGAAATGAAAAATGCCAAAA	GTTCGAGTCCGTATAGCCCT	22	20	36.4	55.0	56.9	58.7	0.0	0.0	3.771615	76885	78001	1224-1235	1.000000	57.5
ycf1_p1	ycf1	1	TCCGTGCTCTCACAACAAAA	AACGACTGCCATTGTTCGTA	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.189093	140538	140657	799-799	0.500000	58.5
ycf1_p2	ycf1	2	GAGATCTTGGATCCGTGCTC	AACGACTGCCATTGTTCGTA	20	20	55.0	45.0	57.9	57.8	7.4	0.0	0.305270	140538	140657	810-810	0.500000	57.6
ycf1_p3	ycf1	3	GATCTTGGATCCGTGCTCTC	AACGACTGCCATTGTTCGTA	20	20	55.0	45.0	57.9	57.8	7.4	0.0	0.305270	140538	140657	808-808	0.500000	57.6
ycf1_p4	ycf1	4	GATCCGTGCTCTCACAACAA	AACGACTGCCATTGTTCGTA	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.362067	140538	140657	801-801	0.500000	57.1
ycf1_p5	ycf1	5	TATTGAGGCCTGGATCACGA	AACGACTGCCATTGTTCGTA	20	20	50.0	45.0	58.2	57.8	12.0	0.0	0.369605	140538	140657	1226-1226	0.500000	57.0
ndhH_p1	ndhH	1	CTTTCAGCAATGCACGGAAG	CCATCTGCTAGAGCTTGCAA	20	20	50.0	50.0	58.0	58.0	8.3	7.9	0.060966	141892	143073	1458-1458	0.500000	59.5
ndhH_p2	ndhH	2	CTTTCAGCAATGCACGGAAG	CATAAGAAGTCCAACGGGGG	20	20	50.0	55.0	58.0	58.0	8.3	0.0	0.065249	141892	143073	1663-1663	0.500000	59.5
ndhH_p3	ndhH	3	CTTTCAGCAATGCACGGAAG	AGAGCTTGCAAAAGTCCCAA	20	20	50.0	45.0	58.0	57.9	8.3	0.0	0.098639	141892	143073	1449-1449	0.500000	59.2
ndhH_p4	ndhH	4	CTTTCAGCAATGCACGGAAG	CGTTGTTGTATCCCTGCAGA	20	20	50.0	50.0	58.0	57.9	8.3	17.4	0.120168	141892	143073	1404-1404	0.500000	59.0
ndhH_p5	ndhH	5	CAGCAATGCACGGAAGAATG	CCATCTGCTAGAGCTTGCAA	20	20	50.0	50.0	58.1	58.0	8.3	7.9	0.129960	141892	143073	1454-1454	0.500000	59.0
ndhD_p1	ndhD	1	TGTTGGGCTGGCTATTGTTT	TTTGTGTGAACTGGCGAGAA	20	20	45.0	45.0	58.0	58.0	0.0	1.4	0.039501	147769	149274	2411-2411	0.500000	59.7
ndhD_p2	ndhD	2	CGGGTTTGATACGGGTAAGG	TTTGTGTGAACTGGCGAGAA	20	20	55.0	45.0	58.1	58.0	0.0	1.4	0.087227	147769	149274	2272-2272	0.500000	59.3
ndhD_p3	ndhD	3	TGTTGGGCTGGCTATTGTTT	TTGGAGTTGGGACCCAAAAG	20	20	45.0	50.0	58.0	57.9	0.0	27.9	0.088896	147769	149274	2700-2700	0.500000	59.3
ndhD_p4	ndhD	4	TGTTGGGCTGGCTATTGTTT	CTAACGAAGCATGGGGATCC	20	20	45.0	55.0	58.0	58.1	0.0	1.9	0.124007	147769	149274	2722-2722	0.500000	59.0
ndhD_p5	ndhD	5	ACAAATCGCTTCTGCTCCAA	TTTGTGTGAACTGGCGAGAA	20	20	45.0	45.0	58.1	58.0	0.0	1.4	0.135039	147769	149274	1963-1963	0.500000	58.9
ccsA_p1	ccsA	1	GCATTACCAGGGATGAGTGG	TCAATTCAAGACAAGCCGCT	20	20	55.0	45.0	58.0	58.1	8.8	0.0	0.130908	149545	150519	1801-1801	0.500000	59.0
ccsA_p2	ccsA	2	TTCTCGCCAGTTCACACAAA	TCAATTCAAGACAAGCCGCT	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.135039	149545	150519	1275-1275	0.500000	58.9
ccsA_p3	ccsA	3	TAGCTTTTCGATGGCTTCCC	TCAATTCAAGACAAGCCGCT	20	20	50.0	45.0	58.0	58.1	11.4	0.0	0.142167	149545	150519	1823-1823	0.500000	58.9
ccsA_p4	ccsA	4	GCATTACCAGGGATGAGTGG	TAGACACGCTGCTCTTAGGA	20	20	55.0	50.0	58.0	57.9	8.8	0.0	0.151405	149545	150519	1768-1768	0.500000	58.8
ccsA_p5	ccsA	5	TTCTCGCCAGTTCACACAAA	TAGACACGCTGCTCTTAGGA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.155536	149545	150519	1242-1242	0.500000	58.8
ndhF_p1	ndhF	1	GGAAAACGCCTTTAACGCTG	TCTTTTCGATTCGAAGCGGT	20	20	50.0	45.0	58.3	57.9	0.0	32.4	0.392722	151871	154129	2865-2865	0.500000	56.9
ndhF_p2	ndhF	2	GATCCATTAGGCCGGAAACA	TCTTTTCGATTCGAAGCGGT	20	20	50.0	45.0	57.7	57.9	5.0	32.4	0.416393	151871	154129	2369-2369	0.500000	56.7
ndhF_p3	ndhF	3	GGAAAACGCCTTTAACGCTG	CTTTTCGATTCGAAGCGGTG	20	20	50.0	50.0	58.3	57.9	0.0	32.4	0.463228	151871	154129	2864-2864	0.500000	56.3
ndhF_p4	ndhF	4	AAAACGCCTTTAACGCTGTC	TCTTTTCGATTCGAAGCGGT	20	20	45.0	45.0	57.6	57.9	0.0	32.4	0.469092	151871	154129	2863-2863	0.500000	56.2
ndhF_p5	ndhF	5	GAAAACGCCTTTAACGCTGT	TCTTTTCGATTCGAAGCGGT	20	20	45.0	45.0	57.6	57.9	0.0	32.4	0.469092	151871	154129	2864-2864	0.500000	56.2