Hotspot

rpoA

LSC · 75556-76566 · 1011 bp

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Pi 0.00415

MarkerSeek score 54.10

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1011
variable_sites: 8
pi: 0.00415
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGTGCTACAGATTGAAAACCT	AATGGCTGTAGACCTCCGAA	38.1	50.0	55.4	58.4	1195 bp	1.0	48.5
rpoA_p2	TGTGCTACAGATTGAAAACCT	ATGGCTGTAGACCTCCGAAA	38.1	50.0	55.4	58.4	1194 bp	1.0	48.5
rpoA_p3	TGTGCTACAGATTGAAAACCT	TAATGGCTGTAGACCTCCGA	38.1	50.0	55.4	57.6	1196 bp	1.0	48.4
rpoA_p4	TGTGCTACAGATTGAAAACCT	GGCTGTAGACCTCCGAAAAA	38.1	50.0	55.4	57.5	1192 bp	1.0	48.2
rpoA_p5	TGTGCTACAGATTGAAAACCT	TGGCTGTAGACCTCCGAAAA	38.1	50.0	55.4	58.7	1193 bp	1.0	46.7