Hotspot

ndhH

SSC · 118596-119777 · 1182 bp

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Pi 0.00321

MarkerSeek score 60.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 9
pi: 0.00321
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CAAGCATCCACAAGATCCCA	GCAATGCATGTAAGAATGACTCA	50.0	39.1	57.9	57.8	1307 bp	1.0	53.6
ndhH_p2	CAAGCATCCACAAGATCCCA	TCAGCAATGCATGTAAGAATGAC	50.0	39.1	57.9	57.8	1310 bp	1.0	53.6
ndhH_p3	ACAAGCATCCACAAGATCCC	GCAATGCATGTAAGAATGACTCA	50.0	39.1	57.9	57.8	1308 bp	1.0	53.6
ndhH_p4	ACAAGCATCCACAAGATCCC	TCAGCAATGCATGTAAGAATGAC	50.0	39.1	57.9	57.8	1311 bp	1.0	53.6
ndhH_p5	CAAGCATCCACAAGATCCCA	CAGCAATGCATGTAAGAATGACT	50.0	39.1	57.9	57.8	1309 bp	1.0	53.5