Hotspot

ndhF

SSC · 107388-109616 · 2229 bp

Back to Linaria

Pi 0.00341

MarkerSeek score 37.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 15
pi: 0.00341
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCGAGTTACAGAGACAGGCT	TGTTCAAAAACGTCTGTGCG	50.0	45.0	57.8	57.8	2869-2876 bp	1.0	77.9
ndhF_p2	AAAAGGCGGATCCGAAGAAG	TGTTCAAAAACGTCTGTGCG	50.0	45.0	58.3	57.8	2918-2925 bp	1.0	77.4
ndhF_p3	AAGAAAAGGCGGATCCGAAG	TGTTCAAAAACGTCTGTGCG	50.0	45.0	58.3	57.8	2921-2928 bp	1.0	77.4
ndhF_p4	GAAAAGGCGGATCCGAAGAA	TGTTCAAAAACGTCTGTGCG	50.0	45.0	58.3	57.8	2919-2926 bp	1.0	77.3
ndhF_p5	GAAGAAAAGGCGGATCCGAA	TGTTCAAAAACGTCTGTGCG	50.0	45.0	58.3	57.8	2922-2929 bp	1.0	77.3