Hotspot

atpB

LSC · 50945-52441 · 1497 bp

Back to Linaria

Pi 0.00254

MarkerSeek score 60.60

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 8
pi: 0.00254
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	GCAATAGGGGCATGATTTGG	GATCGACGTGCTATCAGACA	50.0	50.0	57.2	57.2	1700 bp	1.0	67.7
atpB_p2	GCAATAGGGGCATGATTTGG	TGATCGACGTGCTATCAGAC	50.0	50.0	57.2	57.2	1701 bp	1.0	67.7
atpB_p3	GCAATAGGGGCATGATTTGG	ACTGATCGACGTGCTATCAG	50.0	50.0	57.2	57.2	1703 bp	1.0	67.6
atpB_p4	TCACTTCGGAATCCCAAACA	GATCGACGTGCTATCAGACA	45.0	50.0	57.1	57.2	1636 bp	1.0	66.8
atpB_p5	TCACTTCGGAATCCCAAACA	TGATCGACGTGCTATCAGAC	45.0	50.0	57.1	57.2	1637 bp	1.0	66.8