Hotspot

rpl32-trnL-UAG

SSC · 110367-111018 · 652 bp

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Pi 0.0093
MarkerSeek score 47.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
652
variable_sites
11
pi
0.0093
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGGAAAGGTTGTTGGGCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 784-802 bp 1.0 82.0
rpl32-trnL-UAG_p2 AAAGGAAAGGTTGTTGGGCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 785-803 bp 1.0 82.0
rpl32-trnL-UAG_p3 CGCACAGACGTTTTTGAACA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 920-938 bp 1.0 81.3
rpl32-trnL-UAG_p4 CGCACAGACGTTTTTGAACA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 921-939 bp 1.0 81.3
rpl32-trnL-UAG_p5 ACGCACAGACGTTTTTGAAC AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 921-939 bp 1.0 81.3