Hotspot

ndhF-rpl32

SSC · 109617-110189 · 573 bp

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Pi 0.00489
MarkerSeek score 33.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
573
variable_sites
6
pi
0.00489
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCACGCATATTGATATGTCTGT TGCCCAACAACCTTTCCTTT 39.1 45.0 57.8 58.1 682-683 bp 1.0 54.0
ndhF-rpl32_p2 TCCACGCATATTGATATGTCTGT TTTTAACGCTGCCCAACAAC 39.1 45.0 57.8 57.8 691-692 bp 1.0 53.3
ndhF-rpl32_p3 TCCACGCATATTGATATGTCTGT AGGAAAGGGCTTTTAACGCT 39.1 45.0 57.8 57.7 701-702 bp 1.0 52.7
ndhF-rpl32_p4 TCCACGCATATTGATATGTCTGT GAAAGGGCTTTTAACGCTGC 39.1 50.0 57.8 58.3 699-700 bp 1.0 52.6
ndhF-rpl32_p5 TCCACGCATATTGATATGTCTGT CCCCAAGGAAAGGGCTTTTA 39.1 50.0 57.8 57.7 706-707 bp 1.0 52.4