Hotspot

ndhC-trnV-UAC

LSC · 48356-49425 · 1070 bp

Back to Linaria

Pi 0.00654

MarkerSeek score 49.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1070
variable_sites: 14
pi: 0.00654
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1201-1204 bp	1.0	78.7
ndhC-trnV-UAC_p2	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1200-1203 bp	1.0	75.9
ndhC-trnV-UAC_p3	TCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1214-1217 bp	1.0	75.5
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1189-1192 bp	1.0	74.8
ndhC-trnV-UAC_p5	GGGGCTAAAATCCCGGAAAT	AAGGTCTACGGTTCGAGTCC	50.0	55.0	57.9	58.8	1199-1202 bp	1.0	73.7