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psbM-trnD-GUC

LSC · 28542-29238 · 697 bp

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Pi 0.00402

MarkerSeek score 56.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 6
pi: 0.00402
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGGCTGGCTGTTTTTACGTA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.7	58.5	855-858 bp	1.0	75.1
psbM-trnD-GUC_p2	TTTGGCTGGCTGTTTTTACG	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.5	58.5	857-860 bp	1.0	73.1
psbM-trnD-GUC_p3	TTGGCTGGCTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.6	58.5	856-859 bp	1.0	72.3
psbM-trnD-GUC_p4	TGGCTGGCTGTTTTTACGTA	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.7	57.0	856-859 bp	1.0	70.9
psbM-trnD-GUC_p5	TGGCTGGCTGTTTTTACGTA	TCAATTGGTCAGAGCACCG	45.0	52.6	57.7	57.8	854-857 bp	1.0	69.0