Hotspot

rpoB-trnC-GCA

LSC · 26347-27524 · 1178 bp

Back to Linaria

Pi 0.0056

MarkerSeek score 46.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1178
variable_sites: 14
pi: 0.0056
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TGCTTCATTTGCATCTCCCA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.8	58.1	1264-1268 bp	1.0	78.5
rpoB-trnC-GCA_p2	TTCATTTGCATCTCCCAGCA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.8	58.1	1261-1265 bp	1.0	78.5
rpoB-trnC-GCA_p3	TCATTTGCATCTCCCAGCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.6	58.1	1260-1264 bp	1.0	76.7
rpoB-trnC-GCA_p4	TGCTTCATTTGCATCTCCCA	AAAAGGATTTGCAGTCCCCC	45.0	50.0	57.8	58.4	1246-1250 bp	1.0	76.3
rpoB-trnC-GCA_p5	TTCATTTGCATCTCCCAGCA	AAAAGGATTTGCAGTCCCCC	45.0	50.0	57.8	58.4	1243-1247 bp	1.0	76.3